Highlights• Chlorella species were mutated by 60Co γ irradiation for high CO2 tolerance.• Mutants were domesticated by high concentrations of CO2 for higher growth rate.• Biomass yield of domesticated mutants was 0.5 times higher than original strain.• Enhancing light transmission and culture mixing promoted algae growth rate.• The peak CO2 fixation efficiency of Chlorella PY-ZU1 [...]