In an effort to process wet algal biomass directly, eliminate organic solvent use during lipid extraction, and recover nutrients (e.g., N, P, and glycerol) for reuse, we developed a catalyst-free, two-step technique for algal biodiesel production. In the first step, wet algal biomass (ca. 80% moisture) reacts in subcritical water to hydrolyze intracellular lipids, conglomerate [...]
